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Long non-coding RNA ADAMTS9-AS2 inhibits liver cancer cell proliferation, migration and invasion

Hanjun Li1,2, Hu Huang3,4, Sha Li5

  • 1The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Experimental and Therapeutic Medicine
|April 14, 2021
PubMed

Insights

Long non-coding RNA ADAMTS9-AS2 inhibits liver cancer progression by downregulating the PI3K/AKT/mTOR pathway. This study reveals ADAMTS9-AS2 as a potential therapeutic target for liver cancer, impacting cell proliferation, migration, and invasion.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNA (lncRNA) ADAM metallopeptidase with thrombospondin type 1 motif 9 antisense RNA 2 (ADAMTS9-AS2) is implicated in various cancers.
  • The specific role of ADAMTS9-AS2 in human liver cancer remains largely unelucidated.

Purpose of the Study:

  • To investigate the functional role of ADAMTS9-AS2 in human liver cancer.
  • To explore the underlying molecular mechanisms by which ADAMTS9-AS2 influences liver cancer progression.

Main Methods:

  • Assessed ADAMTS9-AS2 and ADAMTS9 expression levels following overexpression and knockdown.
  • Utilized Cell Counting Kit-8, wound healing, and Transwell assays to evaluate cell proliferation, migration, and invasion.
  • Analyzed protein expression of key signaling molecules (PI3K/AKT/mTOR pathway), autophagy markers (LC3-I/II, BECN1, SQSTM1), and apoptosis regulators (Bax, Bcl-2) via Western blotting.

Main Results:

  • ADAMTS9-AS2 expression positively correlated with ADAMTS9 mRNA and protein levels.
  • ADAMTS9-AS2 significantly inhibited liver cancer cell proliferation, migration, and invasion.
  • ADAMTS9-AS2 downregulated PI3K/AKT/mTOR signaling, reduced SQSTM1 and Bcl-2, and increased LC3-II, BECN1, and Bax expression.

Conclusions:

  • ADAMTS9-AS2 functions as a tumor suppressor in liver cancer.
  • The inhibitory effects of ADAMTS9-AS2 are mediated through the suppression of the PI3K/AKT/mTOR signaling pathway.
  • ADAMTS9-AS2 represents a potential therapeutic target for liver cancer treatment.
Keywords:
PI3K/AKT/mTORautophagyliver cancer cellslncRNA ADAMTS9-AS2proliferation

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