Related Experiment Video

Updated: Nov 9, 2025

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

776

Circular RNA hsa-circ-000881 suppresses the progression of lung adenocarcinoma in vitro via a miR-665/PRICKLE2 axis

Chu Huang1, Weiming Yue1, Lin Li1

  • 1Department of Thoracic Surgery, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Annals of Translational Medicine
|April 14, 2021
PubMed
Abstract

Insights

Circular RNA hsa-circ-000881 inhibits lung adenocarcinoma (LUAD) progression by sponging miR-665 and targeting PRICKLE2. This finding suggests hsa-circ-000881 as a potential therapeutic target for LUAD.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in tumor biology.
  • Many circRNAs influence the progression of lung adenocarcinoma (LUAD).
  • The specific function of hsa-circ-000881 in LUAD remains to be fully elucidated.

Purpose of the Study:

  • To investigate the oncogenic role of hsa-circ-000881 in LUAD.
  • To explore the underlying molecular mechanism of hsa-circ-000881 in LUAD progression.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) and Western blot to assess gene and protein expression.
  • In vitro functional assays to evaluate LUAD cell proliferation, migration, and invasion.
  • Luciferase reporter assays and bioinformatics analysis to confirm molecular interactions.

Main Results:

  • hsa-circ-000881 expression was significantly downregulated in LUAD tissues.
  • Overexpression of hsa-circ-000881 suppressed LUAD cell growth, migration, and invasion.
  • hsa-circ-000881 acts as a molecular sponge for miR-665, with PRICKLE2 identified as a direct target of miR-665.

Conclusions:

  • hsa-circ-000881 exhibits inhibitory effects on LUAD progression through the miR-665/PRICKLE2 pathway.
  • hsa-circ-000881 represents a potential therapeutic target for LUAD treatment.
Keywords:
Circ-000881PRICKLE2lung adenocarcinoma (LUAD)miR-665

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.8K
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.6K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.7K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.8K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.7K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.3K