Sink-source transition in tobacco leaves visualized using chlorophyll fluorescence imaging

•  The sink-source transition of developing Nicotiana tabacum (tobacco) leaves was studied here using chlorophyll fluorescence imaging. •  In accordance with leaf development, the quantum efficiency of PSII, showed a steep gradient across the leaf with increasing values towards the tip. •  The linear electron transport rate (ETR) saturated at higher CO₂ concentrations in the younger, than in the mature, part of the leaf, probably due to a lower Rubisco activity or a higher CO₂ diffusion resistance. •  The induction of ETR at CO₂ concentrations near the compensation point after long-term dark adaptation of the young leaf, showed distinct responses; ETR rose rapidly in the basal but more slowly in the apical regions. There was a correlation between fast induction and carbohydrate import, as measured by ¹⁴ C-translocation. In the basal regions, larger pools of metabolic intermediates are expected due to imported carbohydrates. These might be used in the Calvin cycle directly after dark-light transition providing the electron acceptors for the faster induction of ETR. Additionally, a higher mitochondrial respiration can provide CO₂ for the Calvin cycle in these regions.