Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

6.0K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From pancreas and islet resources to diabetes insights.

Diabetologia·2026
Same author

Heparan sulfate fine-tuned interleukin-1 (IL-1) signaling inhibits insulin secretion of grafted pancreatic islets.

Science advances·2025
Same author

TMEM55A-mediated PI5P signalling regulates alpha cell actin depolymerisation and glucagon secretion.

Diabetologia·2025
Same author

TMEM55A-mediated PI5P signaling regulates α-cell actin depolymerization and glucagon secretion.

bioRxiv : the preprint server for biology·2025
Same author

HumanIslets: An integrated platform for human islet data access and analysis.

bioRxiv : the preprint server for biology·2024
Same author

A role and mechanism for redox sensing by SENP1 in β-cell responses to high fat feeding.

Nature communications·2024

Related Experiment Video

Updated: Nov 9, 2025

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
11:44

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Published on: August 29, 2016

11.2K

Novel mouse model expands potential human α-cell research.

Theodore Dos Santos1,2, Patrick E MacDonald1,2

  • 1Department of Pharmacology, University of Alberta, Edmonton, Alberta, Canada.

Islets
|April 15, 2021
PubMed
Summary

Researchers developed a glucagon-deficient mouse model. This model preserves glucagon-like peptide-1 (GLP-1) and GLP-2 secretion, offering a valuable tool for studying human islet transplantation and glucagon responses in diabetes research.

Keywords:
Animal modelGene editingGlucagonIsletsTransplantationα-cells

More Related Videos

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
10:09

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Published on: May 11, 2015

9.7K
Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery
05:15

Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery

Published on: September 27, 2021

1.4K

Related Experiment Videos

Last Updated: Nov 9, 2025

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
11:44

Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells

Published on: August 29, 2016

11.2K
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
10:09

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Published on: May 11, 2015

9.7K
Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery
05:15

Production of Humanized Mouse via Thymic Renal Capsule Grafting, CD34+ Cells Injection, and Cytokine Delivery

Published on: September 27, 2021

1.4K

Area of Science:

  • Endocrinology
  • Diabetes Research
  • Islet Biology

Background:

  • Glucagon plays a crucial role in glucose homeostasis.
  • Understanding glucagon's function is vital for diabetes research.
  • Current models often lack the ability to study specific aspects of glucagon physiology.

Purpose of the Study:

  • To create a novel mouse model for studying glucagon's role in metabolic diseases.
  • To investigate human islet transplantation and glucagon secretion.
  • To provide a resource for advancing diabetes research.

Main Methods:

  • Generation of a glucagon knock-out mouse model.
  • Assessment of preserved glucagon-like peptide-1 (GLP-1) and glucagon-like peptide-2 (GLP-2) secretion.
  • Evaluation of the model's utility in studying transplanted human islets.

Main Results:

  • Successfully generated a glucagon knock-out mouse.
  • Confirmed preserved GLP-1 and GLP-2 secretion in the absence of glucagon.
  • Demonstrated the model's suitability for studying human islet transplantation and glucagon responses.

Conclusions:

  • The developed glucagon knock-out mouse is a significant advancement for diabetes research.
  • This model facilitates improved studies of human islet transplantation.
  • It offers unprecedented insights into human alpha-cell function and glucagon's role in metabolic regulation.