Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterization of a lytic polysaccharide monooxygenase important for utilization of chitin in Chitinibacter mangrovi FCG-7.

International journal of biological macromolecules·2026

Same author

One-step enzymatic synthesis of medium molecular weight dextran using engineered dextransucrase DarM from <i>Leuconostoc citreum</i> CBA3623.

Frontiers in microbiology·2026

Same author

Rational Engineering of a GH18 Chitinase for Enhanced Thermostability and Catalytic Efficiency via Dynamic Allosteric Modulation.

Journal of agricultural and food chemistry·2026

Same author

Switching of biodetoxification stage to microbial lipid fermentation stage by adding fungicide sodium hypochlorite in lignocellulosic biorefining process.

Bioprocess and biosystems engineering·2026

Same author

Atmospheric room-temperature plasma mutagenesis and heterologous expression of dextranase in Aspergillus niger.

AMB Express·2026

Same author

Highly efficient chitinase production from <i>Chitinibacter mangrovi</i> FCG-7<sup>T</sup> and immunomodulatory efficacy of generated GlcNAc in cyclophosphamide-induced immunosuppression.

Frontiers in microbiology·2026

Same journal

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026

Same journal

Convergence and conflict among telomere specialized transposons across 60 million years of Drosophilid evolution.

Genome research·2026

Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026

Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026

Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026

Same journal

Spatially informed reference-free cell-type deconvolution for spatial transcriptomics with SpatialCD.

Genome research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Nov 9, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

Profiling single-cell histone modifications using indexing chromatin immunocleavage sequencing.

Wai Lim Ku¹, Lixia Pan¹, Yaqiang Cao¹

¹Laboratory of Epigenome Biology, Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892-1674, USA.

Genome Research

|April 15, 2021

Summary

This summary is machine-generated.

We developed indexing single-cell immunocleavage sequencing (iscChIC-seq) to analyze histone modifications in tens of thousands of single cells. This method overcomes limitations of previous techniques, enabling deeper insights into cellular heterogeneity.

More Related Videos

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells

Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells

Published on: December 10, 2014

Related Experiment Videos

Last Updated: Nov 9, 2025

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin

Published on: April 11, 2014

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells

Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells

Published on: December 10, 2014

Area of Science:

Epigenetics
Single-cell biology
Genomics

Background:

Existing single-cell assays for histone marks have low throughput or sparse data.
This limits their application in complex biological systems.

Purpose of the Study:

To introduce indexing single-cell immunocleavage sequencing (iscChIC-seq) for high-throughput single-cell histone modification analysis.
To demonstrate the capability of iscChIC-seq in profiling histone modifications across tens of thousands of cells.

Main Methods:

Developed a multiplex indexing method using TdT terminal transferase and T4 DNA ligase-mediated barcoding.
Applied iscChIC-seq to profile H3K4me3 and H3K27me3 in human white blood cells (WBCs).

Main Results:

Successfully analyzed over 10,000 single cells for each histone modification.
Achieved high nonredundant reads per cell (11K for H3K4me3, 45K for H3K27me3).
Identified distinct immune cell populations (monocytes, T cells, B cells, NK cells) based on histone mark profiles, with correlated annotations between H3K4me3 and H3K27me3 data.

Conclusions:

iscChIC-seq is a reliable technique for large-scale single-cell histone modification profiling.
This method facilitates the study of cellular heterogeneity and differentiation in complex systems.
Potential broad applications in developmental biology and disease research.