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Cell Migration

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.

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Related Experiment Video

Updated: Nov 9, 2025

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Published on: April 4, 2013

An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration.

Meng Sun¹, Bence Rethi¹, Akilan Krishnamurthy¹

¹Rheumatology Unit, Department of Medicine, Karolinska Insititutet, Stockholm, Sweden.

|April 15, 2021

Summary

This protocol enhances cell migration monitoring using live-cell imaging and automated analysis. It offers a reliable and reproducible method for studying cell mobility and treatment effects.

Keywords:

Cell migration High-throughput Live cell image Scratching assay Time-lapse imaging

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Related Experiment Videos

Last Updated: Nov 9, 2025

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Published on: April 4, 2013

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Published on: March 7, 2025

Area of Science:

Cell Biology
Biotechnology

Background:

Scratch assays are standard for studying cell migration and wound healing.
Traditional scratch assays face challenges in reproducibility due to manual variability in scratching, analysis timing, and image interpretation.

Purpose of the Study:

To present a refined protocol for monitoring cell migration using live-cell imaging.
To overcome limitations of conventional scratch assays by implementing homogenous wounding, sequential imaging, and automated analysis.

Main Methods:

Utilized adherent cells cultured in 96-well plates.
Performed homogenous linear scratches using the IncuCyte® WoundMaker.
Acquired sequential images every 2 hours and analyzed using IncuCyte® Cell Migration Analysis Software with automated masking.

Main Results:

The protocol enables time-lapse analysis of cell migration.
Automated image analysis ensures objective assessment of cell mobility.
Demonstrates a highly reliable and reproducible method for studying treatment effects on cell migration.

Conclusions:

This protocol provides a robust and reproducible method for monitoring cell migration.
The automated approach minimizes variability, enhancing the reliability of cell migration studies.
Facilitates accurate, time-lapse assessment of treatment impacts on cell mobility.