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Updated: Nov 9, 2025
In vivo Visualization of Synaptic Vesicles Within Drosophila Larval Segmental Axons
Published on: October 15, 2010
Investigate Synaptic Vesicles Mobility in Neuronal Culture Axons by FRAP Imaging
Xiao Min Zhang1, Fabrice P Cordelieres2, Etienne Herzog3
1Department of Pathophysiology, Guangdong Provincial Key Laboratory of Brain Function and Disease, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
Abstract:
Synaptic vesicles (SVs) are clustered in the presynaptic terminals and consistently trafficking along axons. Based on their release features, SVs are classified into different "pools". Imaging of SVs that are traveling among multiple presynaptic terminals has helped define a new pool named "SV super-pool". Here we describe a Fluorescent Recovery After Photobleaching (FRAP) approach to elucidate the relationship between SVs from the super-pool with SV clusters at presynaptic terminals. This method is powerful to investigate SV mobility regulation mechanisms.
