Related Experiment Video
Updated: Nov 9, 2025
08:42
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
Published on: July 3, 2020
4.7K
miR-146a-5p targets Sirt1 to regulate bone mass
Mingxia Zheng1, Junlong Tan1, Xiangning Liu1
1Clinical Research Platform for Interdiscipline of Stomatology, The First Affiliated Hospital of Jinan University & Department of Stomatology, College of Stomatology, Jinan University, Guangdong 510632, China.
Bone Reports
|April 15, 2021
Summary
MicroRNAs (miRNAs) regulate bone formation. This study identifies miR-146a-5p as a suppressor of osteoblastogenesis, targeting Sirt1 and potentially offering a therapeutic strategy for osteoporosis.
Area of Science:
- Molecular Biology
- Bone Biology
- Genetics
Background:
- MicroRNAs (miRNAs) are key post-transcriptional regulators impacting biological processes.
- miR-146a-5p influences osteoblast and osteoclast activity, but its specific targets in bone formation were unknown.
Purpose of the Study:
- To identify the role and target genes of miR-146a-5p in osteoblastogenesis and bone formation.
- To investigate the potential of targeting miR-146a-5p for osteoporosis treatment.
Main Methods:
- Utilized miR-146a-5p knockout mice for in vivo studies.
- Performed in vitro studies using bone marrow mesenchymal stem cells (BMSCs).
- Investigated the direct interaction between miR-146a-5p and Sirt1.
Main Results:
- miR-146a-5p knockout mice showed increased bone formation and mass.
- miR-146a-5p inhibited osteoblast differentiation in vitro.
- miR-146a-5p directly targets Sirt1, suppressing osteoblast activity.
- miR-146a-5p expression increased with age in mice and human patients, contributing to bone loss.
Conclusions:
- miR-146a-5p is a critical suppressor of osteoblastogenesis and bone formation.
- miR-146a-5p deletion protects against age-related bone loss.
- Inhibiting miR-146a-5p represents a potential therapeutic strategy for osteoporosis.
Related Concept Videos
01:17Hormones and Bone Tissue
3.3K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.3K
01:22PI3K/mTOR/AKT Signaling Pathway
4.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
4.4K
01:40Bone Remodeling
39.3K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
39.3K
03:03mTOR Signaling and Cancer Progression
4.0K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.0K
01:31Osteoclasts in Bone Remodeling
3.5K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.5K