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Dynamic post-transcriptional regulation by Mrn1 links cell wall homeostasis to mitochondrial structure and function

Kendra Reynaud1, Molly Brothers2, Michael Ly2

  • 1California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, California, United States of America.

Plos Genetics
|April 15, 2021
PubMed

Insights

The RNA-binding protein Mrn1 regulates yeast cell wall and mitochondrial genes. Loss of Mrn1 enhances adaptation to respiratory conditions by altering mitochondrial structure.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Cellular Respiration

Background:

  • The RNA-binding protein Mrn1 in Saccharomyces cerevisiae regulates over 300 messenger RNAs.
  • Mrn1's impact on target transcripts and its cellular role in regulation are not fully understood.
  • Mrn1 targets include SUN domain proteins Nca3 and Uth1, affecting mitochondrial and cell wall functions.

Purpose of the Study:

  • To investigate the regulatory impact of Mrn1 on its target mRNAs.
  • To elucidate the cellular role of Mrn1-mediated gene regulation.
  • To understand how Mrn1 integrates cell wall integrity and mitochondrial biosynthesis.

Main Methods:

  • Analysis of Mrn1's N-terminal domain for regulatory function.
  • Identification and study of endogenous Mrn1 targets, including Nca3 and Uth1.
  • Phenotypic analysis of mrn1Δ yeast under fermentative and respiratory conditions.

Main Results:

  • Mrn1 represses target mRNAs via its disordered, asparagine-rich amino-terminus.
  • Loss of MRN1 leads to faster adaptation to respiratory conditions.
  • mrn1Δ cells exhibit enlarged mitochondria under fermentative conditions, partly due to NCA3 dysregulation.

Conclusions:

  • Mrn1 acts as a regulatory hub integrating cell wall integrity and mitochondrial biosynthesis.
  • Mrn1-mediated regulation is responsive to carbon source availability.
  • Dysregulation of Mrn1 affects mitochondrial morphology and respiratory adaptation in yeast.

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