Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin01:26

Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin

886
Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...
886
Physical Properties of Amines01:26

Physical Properties of Amines

3.7K
Amines with low molecular weight are usually gaseous at room temperature, while those with high molecular weight are liquid or solids in nature. Usually, low molecular weight amines have a rotten fish-like smell. Diamines typically have a pungent smell. For instance, cadaverine and putrescine, depicted in Figure 1, are two molecules responsible for decaying tissue.
3.7K
Local Anesthetics: Differential Sensitivity of Nerve Fibers01:24

Local Anesthetics: Differential Sensitivity of Nerve Fibers

1.1K
Local anesthetics (LAs) block the sodium channels of nerve trunks, sensory nerve endings, and neuromuscular junctions. Although LAs can block all kinds of nerves, the sensitivity of nerve fibers differs according to nerve types and structures. LAs are known to block myelinated fibers faster than unmyelinated ones. Also, they block pain or sensory neurons at low concentrations without affecting the motor neurons involved in muscle contractions. This helps relieve labor pain without affecting the...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to "Structure and Absolute Configuration of Diterpenoids from <i>Hymenaea stigonocarpa</i>".

Journal of natural products·2026
Same author

A Multidimensional Evaluation of Sakuranetin against <i>Schistosoma mansoni</i>: From Drug-Likeness to <i>In Vivo</i> Efficacy.

ACS omega·2026
Same author

Continuous-Flow Asymmetric Photocatalysis for the Synthesis of Natural and Unnatural α‑Amino Acid Derivatives.

ACS organic & inorganic Au·2026
Same author

The Metabolome of the <i>Ocotea</i> spp.: From Biosynthetic Aspects to Bioactive Chemical Scaffolds by Integrating the Genus Chemical Database (<i>Ocotea</i>DB).

Journal of natural products·2026
Same author

Spathulenol, a Sesquiterpene from <i>Guarea macrophylla</i>, Displays Potent and Selective <i>Anthelmintic</i> Activity against <i>Angiostrongylus cantonensis</i>.

ACS omega·2026
Same author

Computational modeling-assisted discovery of the structural effects of phenylpropanoid derivatives on intracellular anti-Trypanosoma cruzi activity.

Bioorganic & medicinal chemistry letters·2026

Related Experiment Video

Updated: Nov 9, 2025

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

1.2K

Antitrypanosomal Lactones from Nectandra barbellata.

Vinicius S Londero1, Thais A Costa-Silva2, Guilherme M Antar3

  • 1Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, São Paulo 05508-000, Brazil.

Journal of Natural Products
|April 15, 2021
PubMed
Summary

Researchers isolated novel lactones from Nectandra barbellata using microwave-assisted extraction. These compounds showed activity against Trypanosoma cruzi, suggesting potential for new anti-parasitic drug development.

More Related Videos

Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes
07:08

Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes

Published on: December 22, 2013

4.9K
An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity
12:02

An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

Published on: November 2, 2016

12.3K

Related Experiment Videos

Last Updated: Nov 9, 2025

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
09:36

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques

Published on: March 8, 2024

1.2K
Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes
07:08

Isolation of Viable Multicellular Glands from Tissue of the Carnivorous Plant, Nepenthes

Published on: December 22, 2013

4.9K
An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity
12:02

An Efficient Method for the Synthesis of Peptoids with Mixed Lysine-type/Arginine-type Monomers and Evaluation of Their Anti-leishmanial Activity

Published on: November 2, 2016

12.3K

Area of Science:

  • Natural Product Chemistry
  • Medicinal Chemistry
  • Parasitology

Background:

  • Nectandra barbellata is a plant species with potential for novel bioactive compound discovery.
  • Trypanosoma cruzi is the causative agent of Chagas disease, necessitating new therapeutic strategies.

Purpose of the Study:

  • To isolate and characterize new lactones from Nectandra barbellata.
  • To evaluate the anti-trypanosomal activity of isolated compounds against Trypanosoma cruzi.
  • To investigate the mechanism of action of the bioactive compounds.

Main Methods:

  • Microwave-assisted extraction (MAE) using ionic liquid (BMImBr) in water.
  • Successive chromatographic separation for compound isolation.
  • Structural elucidation using spectroscopic techniques (IR, UV, HRESIMS, NMR, ECD, VCD).
  • In vitro anti-trypanosomal assays and mechanistic studies (membrane potential, ROS, ATP, Ca2+, pH).
  • In silico analysis for pan assay interference compounds (PAINS).

Main Results:

  • Isolation of one sesquiterpene (costic acid) and three new lactones (compounds 1-3).
  • Compounds 1-3 exhibited activity against trypomastigote forms of Trypanosoma cruzi.
  • Mechanistic studies revealed alterations in plasma membrane potential, ROS levels, mitochondrial potential, and acidocalcisome pH.
  • In silico analysis indicated no PAINS resemblance, suggesting potential for drug development.

Conclusions:

  • Nectandra barbellata yielded novel lactones with significant anti-trypanosomal activity.
  • The isolated compounds target key cellular processes in Trypanosoma cruzi.
  • These findings support the development of Nectandra barbellata-derived compounds as leads for Chagas disease therapeutics.