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Related Experiment Video

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Monitoring Protein Adsorption with Solid-state Nanopores
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Selective target protein detection using a decorated nanopore into a microfluidic device.

Izadora Mayumi Fujinami Tanimoto1, Benjamin Cressiot2, Nathalie Jarroux3

  • 1Université Paris-Saclay, Univ Evry, CNRS, LAMBE, 91025, Evry-Courcouronnes, France; Université Paris-Saclay, ENS Paris-Saclay, CNRS, LuMIn, Institut d'Alembert, 91190, Gif-sur-Yvette, France.

Biosensors & Bioelectronics
|April 15, 2021
PubMed
Summary

This study introduces a polymer surface modification for solid-state nanopores, enhancing biosensor stability and enabling precise control over molecular interactions for improved single-molecule analysis. This advancement paves the way for advanced diagnostics.

Keywords:
MicrofluidicsNanopore transportPolymer functionalizationProtein sensingSolid-state nanopore

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Area of Science:

  • Nanotechnology and Materials Science
  • Biophysics and Single-Molecule Analysis
  • Biosensor Development

Background:

  • Solid-state nanopores are crucial for single-molecule analysis but suffer from nonspecific interactions, limiting specific detection.
  • Controlled surface chemistry is essential for reliable nanopore biosensor performance.
  • Existing methods struggle with nonspecific binding, hindering precise biomolecular analysis.

Purpose of the Study:

  • To develop a polymer surface modification for solid-state nanopores to improve stability and enable specific biomolecular detection.
  • To investigate the impact of surface functionalization on nanopore characteristics, including diameter, stability, and ionic conduction.
  • To demonstrate proof-of-concept for specific analyte capture using functionalized nanopores.

Main Methods:

  • Polymer surface modification of solid-state nanopores to passivate the membrane.
  • Characterization of nanopore stability, ionic conduction, and diameter control.
  • Probing the effects of ionic strength and pH on nanopore electroosmotic dynamics.
  • Integration of nanopore chips into microfluidic devices for ease of handling.
  • Experimental validation using biotin-streptavidin binding for specific capture.

Main Results:

  • Polymer functionalization significantly enhances nanopore stability and ionic conduction.
  • Precise control over nanopore diameter and specific protein-surface interactions is achieved.
  • Ionic strength and pH modulate electroosmotic driving force and dynamics.
  • Demonstrated specific capture of streptavidin via grafted biotin, validating the functionalization approach.
  • A model for ionic conductance through a permeable pore surface was elucidated.

Conclusions:

  • Polymer surface modification offers a robust strategy for creating stable and specific solid-state nanopore biosensors.
  • This approach allows for fine-tuning of nanopore properties and analyte interactions, advancing single-molecule analysis.
  • The developed technology holds promise for future applications in virus diagnostics, nanoparticle sensing, and biomarker detection.