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Updated: Nov 9, 2025
Author Spotlight: High-Resolution 4D Light-Sheet Imaging and Virtual Reality in Zebrafish for Single-Cell Analysis of Heart Function
Published on: January 5, 2024
In-vivo 3D imaging of Zebrafish's intersegmental vessel development by a bi-directional light-sheet illumination
Xiaofei Qin1, Chong Chen2, Linbo Wang2
1Changchun University of Science and Technology, Changchun, Jilin, 130022, China.
Abstract:
Precise quantification of vascular developments in Zebrafish requires continuous in-vivo 3D imaging. Here we employed a bi-directional light-sheet illumination microscope to characterize the development process of Zebrafish's intersegmental vessels. A Virtual Reality-based method was used to measure the lengths of intersegmental vessels (ISVs). The quantified growth rates of typical ISVs can be plotted, and unusual growth of some specific vessels was also observed.
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