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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

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Related Experiment Video

Updated: Nov 9, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

A novel tissue specific alternative splicing variant mitigates phenotypes in Ets2 frame-shift mutant models.

Yuki Kishimoto¹, Iori Nishiura¹, Wataru Hirata¹

¹Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara, 630-0192, Japan.

Scientific Reports

|April 16, 2021

Summary

New Ets2 mutant mice generated via CRISPR/Cas9 editing show exon skipping, not previously observed phenotypes. This suggests novel splicing variants can influence gene function analysis and reveal new biological mechanisms.

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Last Updated: Nov 9, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

Genetics
Developmental Biology
Molecular Biology

Background:

Ets2 is crucial for biological processes, with a previously identified mutant (Ets2^db1/db1) causing embryonic lethality due to trophoblast defects.
Tetraploid complementation rescued the Ets2^db1/db1 phenotype, yielding pups with distinct wavy hair and curly whiskers.

Purpose of the Study:

To generate and characterize novel Ets2 mutant models using CRISPR/Cas9 with a frame-shift mutation in exon 8.
To investigate the phenotypic consequences of this new mutation and compare them to existing models.

Main Methods:

CRISPR/Cas9 genome editing to introduce a frame-shift mutation in Ets2 exon 8.
Tetraploid complementation to rescue embryonic lethality.
Analysis of mRNA splicing variants and protein expression in mutant mice.

Main Results:

Homozygous Ets2 mutants generated by natural mating exhibited embryonic lethality before E8.5, consistent with previous findings.
Tetraploid complementation rescue of the new Ets2 mutants did not result in wavy hair or curly whiskers.
Exon 8 skipping was observed, leading to in-frame mutant mRNA and protein expression in skin and thymus, but not early embryos.

Conclusions:

Novel splicing variants, such as exon 8 skipping, can arise from genome editing and complicate phenotypic analysis.
The exon 8-skipped Ets2 mutant protein appears to retain some Ets2 function, particularly in the skin.
These findings highlight the importance of considering unintended splicing alterations in genome editing studies and offer insights into Ets2 gene function.