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A guide to interrogating immunometabolism.

Kelsey Voss¹, Hanna S Hong^2,3, Jackie E Bader¹

¹Vanderbilt Center for Immunobiology, Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.

Nature Reviews. Immunology

|April 16, 2021

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This review offers a framework for immunologists to explore cellular metabolism and immunity. It guides researchers in selecting biochemical techniques and interpreting results to understand the role of metabolism in immune responses.

Area of Science:

Immunology
Biochemistry
Cellular Metabolism

Background:

Metabolic pathways are crucial for immune cell function, impacting energy production, synthesis, signaling, and survival.
Immunologists often encounter metabolic phenotypes but lack guidance on appropriate investigation techniques.
Understanding immunometabolism is essential for advancing research in cellular metabolism and immunity.

Purpose of the Study:

To provide a framework for selecting and applying biochemical techniques to study immunometabolism.
To guide researchers in developing and testing metabolic hypotheses in immunology.
To offer insights and strategies for navigating the field of cellular metabolism and immunity.

Main Methods:

Review of biochemical and metabolomic approaches for studying immunometabolism.

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Discussion of isotope tracing and genetic methodologies.

Evaluation of the strengths and weaknesses of various techniques.

Main Results:

A structured approach to investigating immunometabolism is proposed.
Recommendations for initial hypothesis testing and avoiding common errors are provided.
Recent studies highlighting the impact of metabolic approaches in immunology are showcased.

Conclusions:

The review equips researchers with strategies to confidently explore cellular metabolism and immunity.
It aims to demystify immunometabolism for both new and experienced investigators.
By following the proposed framework, researchers can rigorously uncover the role of metabolism in immune functions.