Surface Characterization and Biocompatibility of Hydroxyapatite Coating on Anodized TiO₂ Nanotubes via PVD Magnetron...

Hydroxyapatite (HA) coating has received significant attention in the scientific community for the development of implants, and HA coating on titanium oxide (TiO₂) nanotubes has shown potential benefits in the improvement of cell proliferation, adhesion, and differentiation. In this study, a HA coating on a TiO₂ nanotubular surface was developed to improve the biocompatibility of the titanium (Ti) surface via magnetron sputtering. Scanning electron microscopy (SEM), surface profilometry, and water contact goniometry revealed that HA-coated TiO₂ nanotubes influenced the surface roughness (R_a) and hydrophilicity. The XRD and FTIR peaks indicated the presence of crystalline phases of TiO₂ (anatase) and HA-coated TiO₂ nanotubes after annealing at 500 °C for 120 min. The HA-coated TiO₂ nanotubes showed significantly increased R_a and decreased water contact angle (θ) compared to the as-anodized TiO₂ nanotubular and bare CP-Ti surfaces. MTS assay using osteoblast-like cells confirmed that the HA-coated TiO₂ nanotubular surface provided an enhanced cell attachment and growth when compared to as-anodized TiO₂ nanotubular and pure CP-Ti surfaces.