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Plasma corticosteroid patterns in the fetus.

K Nahoul1, F Daffos, F Forestier

  • 1Fondation de Recherche en Hormonologie, Fresnes, France.

Journal of Steroid Biochemistry
|June 1, 1988
PubMed
Summary
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Fetal steroid levels, including cortisol and cortisone, were measured in umbilical cord blood. Findings suggest increasing fetal metabolism of steroid precursors and potential for prenatal diagnosis of steroidogenesis disorders.

Area of Science:

  • Endocrinology
  • Perinatal Medicine
  • Biochemistry

Background:

  • Fetal steroidogenesis is crucial for development.
  • Understanding cortisol and its precursors in utero is important.
  • Previous studies have limited data on specific steroid profiles during gestation.

Purpose of the Study:

  • To quantify cortisol (F), cortisone (E), 17-hydroxyprogesterone (17-OHP), and 11-deoxycortisol (S) in fetal umbilical vein blood.
  • To analyze the developmental patterns of these steroids between 19 and 31 weeks of gestation.
  • To explore the metabolic relationships and correlations between these fetal steroids.

Main Methods:

  • Radioimmunoassay of plasma extracts from 137 fetal umbilical vein blood samples.
  • Column chromatography using Sephadex LH-20 for steroid separation.

Related Experiment Videos

  • Analysis of steroid levels across gestational ages from 19 to 31 weeks.

    • Main Results:

    • Cortisol (F) levels remained stable, while cortisone (E) levels increased with gestational age.
    • 11-deoxycortisol (S) and 17-hydroxyprogesterone (17-OHP) levels showed a declining pattern, suggesting increased fetal metabolism.
    • Cortisone (E) correlated significantly with cortisol (F), while 17-OHP and S correlated with each other and cortisol (F).

    Conclusions:

    • Fetal steroid profiles change significantly during the second and third trimesters.
    • The observed patterns indicate increasing fetal utilization and metabolism of cortisol precursors.
    • The described method shows promise for the prenatal diagnosis of congenital disorders of steroid biosynthesis.