Probe with large Stokes shift for effective cysteine imaging in living cells

A new fluorescence probe L, which featured with a large Stokes shift (216 nm), was designed for sensitive detection of cysteine (Cys) and a potential sensing mechanism derived from excited state intramolecular proton transfer (ESIPT) was proposed. More importantly, probe L exhibits higher selective to Cys than other amino acid due to its specific cyclization between acrylate group and Cys. Meanwhile, the probe L shows a low detection limit of 8.82 × 10^-8 M, which is enough for detecting Cys in organisms. Furthermore, this probe displays high biocompatibility and can image Cys in living cells.