Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sulfated Hyaluronan in Dermatology: What's New? Overview of Evidence in Specific Dermatological Diseases.

Pharmaceutics·2025

Same author

Radical-free photopolymerizable composites of hyaluronic acid and gelatin for tissue engineering.

Acta biomaterialia·2025

Same author

Effect of intra-articular injection of a hyaluronic acid-alendronate conjugate on post-traumatic osteoarthritis induced by destabilization of the medial meniscus in rats.

Scientific reports·2023

Same author

Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances.

Gels (Basel, Switzerland)·2023

Same author

A Photopolymerizable Biocompatible Hyaluronic Acid Hydrogel Promotes Early Articular Cartilage Repair in a Minipig Model In Vivo.

Advanced healthcare materials·2023

Same author

Aerosolized sulfated hyaluronan derivatives prolong the survival of K18 ACE2 mice infected with a lethal dose of SARS-CoV-2.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2023

Search research articles

Related Experiment Video

Updated: Nov 9, 2025

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

A versatile and robust analytical method for hyaluronan quantification in crosslinked products and complex matrices.

Matteo Soato¹, Devis Galesso¹, Riccardo Beninatto¹

¹Fidia Farmaceutici, Abano Terme (PD), Italy.

Carbohydrate Research

|April 16, 2021

Summary

A new HPLC method quantifies hyaluronic acid (HA) and its derivatives. This technique works even for modified HA in complex medical devices and supplements, overcoming limitations of standard analytical methods.

Keywords:

Deacetylation Glucosamine Hyaluronan Medical devices Viscosupplement

More Related Videos

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

Related Experiment Videos

Last Updated: Nov 9, 2025

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining

Published on: February 25, 2021

A Quantitative Glycomics and Proteomics Combined Purification Strategy

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

Area of Science:

Biochemistry
Analytical Chemistry
Polymer Science

Background:

Hyaluronic acid (HA) is a biopolymer crucial in pharmaceuticals, medical devices, nutraceuticals, and cosmetics.
Standard analytical methods struggle to identify or quantify HA, especially when chemically modified or in complex matrices.

Purpose of the Study:

To develop and validate a specific, reliable method for quantifying hyaluronic acid (HA) and its derivatives.
To address the analytical challenges posed by modified HA and HA in complex formulations.

Main Methods:

Developed a method involving strong acid hydrolysis, depolymerization, and deacetylation of HA.
Quantitatively yielded glucosamine residues were derivatized with Fluorenylmethyloxycarbonyl chloride (FMOC).
Separation and quantification were performed using High-Performance Liquid Chromatography (HPLC) with UV detection.

Main Results:

The developed HPLC-UV method successfully quantified HA and its derivatives.
The method demonstrated effectiveness on various viscosupplements containing modified HA.
It was also successfully applied to medical devices with unmodified HA in complex matrices.

Conclusions:

A robust analytical method for HA and derivative quantification has been established.
This method overcomes previous limitations, enabling accurate analysis in diverse and complex samples.
The validated technique supports quality control for HA-based products.