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Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
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In Situ Programming of CAR T Cells.
Neha N Parayath1, Matthias T Stephan1,2
1Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
Annual Review of Biomedical Engineering
|April 17, 2021
Summary
In vivo chimeric antigen receptor (CAR) T cell programming offers a promising alternative to complex ex vivo manufacturing. This approach could make advanced gene therapies more accessible for treating various diseases.
Area of Science:
- Biotechnology
- Immunotherapy
- Gene Therapy
Background:
- Chimeric antigen receptor (CAR) T cell therapy is a powerful tool for disease treatment.
- Current CAR T cell manufacturing is complex, expensive, and limits patient access.
Purpose of the Study:
- To assess the clinical need for in situ CAR T cell programming.
- To compare technologies for in vivo T cell engineering.
- To review the progress and future impact of this biotechnology.
Main Methods:
- Literature review and technology comparison.
- Evaluation of clinical requirements for in situ programming.
- Analysis of current research and development in the field.
Main Results:
- In situ CAR T cell programming presents a viable solution to ex vivo manufacturing challenges.
- Several competing technologies are emerging for in vivo T cell engineering.
- The field is rapidly advancing with significant potential.
Conclusions:
- In situ CAR T cell programming holds the potential to broaden access to advanced immunotherapies.
- Continued research and development are crucial for realizing the full impact of this technology.
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