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Human platelet serotonin content: methodological aspects and physiological variations.

P Guicheney1

  • 1INSERM U7, Department of Pharmacology, Hôpital Necker, Paris, France.

Methods and Findings in Experimental and Clinical Pharmacology
|April 1, 1988
PubMed
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This study optimized methods for measuring platelet serotonin (5-HT) using HPLC. Platelet 5-HT levels vary with age and sex, potentially linked to estrogen in women.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Platelet serotonin (5-HT) is a key neurotransmitter with implications in various physiological processes.
  • Accurate quantification of platelet 5-HT is crucial for research and clinical applications.

Purpose of the Study:

  • To establish and validate a reliable method for determining platelet serotonin content.
  • To investigate methodological factors affecting 5-HT measurement.
  • To explore physiological variations in platelet 5-HT levels.

Main Methods:

  • Platelet-rich plasma (PRP) from volunteers was used for serotonin determination.
  • High-performance liquid chromatography (HPLC) with electrochemical detection was employed after perchloric acid lysis.
  • Methodological aspects including anticoagulation, sample storage, and deproteinization were optimized.

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Main Results:

  • EDTA and ACD-C anticoagulation yielded identical results; samples were stable for 2 hours at room temperature.
  • Deproteinized samples were stable for 48 hours at 4°C or weeks at -80°C, with degradation proportional to an internal standard.
  • Mean platelet 5-HT in healthy adults (20-60 years) was 0.364 ± 0.093 nmol/10^8 platelets, with lower levels in men and older women, correlated with plasma estrogen.

Conclusions:

  • A robust HPLC method for platelet 5-HT quantification was developed, allowing for sample transport and extended storage.
  • Platelet serotonin levels exhibit physiological variations influenced by age and sex, with a potential link to hormonal status in women.