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Updated: Nov 9, 2025

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution
Published on: December 30, 2021
Massilia aromaticivorans sp. nov., a BTEX Degrading Bacterium Isolated from Arctic Soil
Jigwan Son1, Hyosun Lee1, Mincheol Kim2
1Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Abstract:
A novel BTEX degrading bacterial strain, designated ML15P13T, was isolated from Arctic soil at the Svalbard Islands, Norway, using an enrichment culture technique. This isolate is Gram-negative, aerobic, motile with multiple flagella at one polar end, and rod-shaped. Growth was observed at 4-35 °C, pH 6.0-8.0, and 0-0.5% (w/v) NaCl. According to 16S rRNA gene analysis, strain ML15P13T was grouped with members of the genus Massilia and closely related to Massilia atriviolacea SODT (98.4%), Massilia violaceinigra B2T (98.3%), Massilia eurypsychrophila B528-3T (97.7%), Massilia glaciei B448-2T (97.7%), and Massilia psychrophila B115-1T (96.6%). Average nucleotide identity, digital DNA-DNA hybridization, and average amino acid identity between genome sequences of strain ML15P13T and the closely related species ranged from 75.8 to 84.3%, from 19.6 ± 1.0 to 21.6 ± 0.3%, and from 68.8 to 71.0%, respectively. The major fatty acids were C16:0, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). Q-8 was the major ubiquinone. The polar lipid profile showed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, and five unidentified polar lipids. The G + C content of the genomic DNA was 64.2 mol%. Based on the results for genotypic and phenotypic study, we conclude that strain ML15P13T represents a novel species of the genus Massilia, for which the name Massilia aromaticivorans sp. nov. is proposed. The type strain is ML15P13T (= KACC 21773T = JCM 34089T).
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