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Fabrication of polydopamine-based NIR-light responsive imprinted nanofibrous membrane for effective lysozyme
Noha Amaly1, Ahmed Y El-Moghazy1, Gang Sun2
1Department of Biological and Agricultural Engineering, University of California, Davis, CA 95616, USA; Polymeric Materials Research Department, Advanced Technology and New Materials Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, Alexandria, Egypt.
Abstract:
The development of highly efficient performance matrix for protein adsorption and scalable throughput adsorbent is highly desired, especially in pharmaceuticals and food industries. In this work, we present a simple methodology to prepare a nanofibrous membrane based surface molecular imprinted matrix (MIP) for selective separation of lysozyme. The MIP was developed by coating carboxylated poly (vinyl alcohol-co-ethylene) nanofibrous mat (EVOH-CCA NFM) with a near infrared (NIR)-light responsive polydopamine (PDA) layer. The open porous nanofibrous structure and a thin PDA layer endowed the MIPs with adsorption capacity (500 mg.g-1) within 150 min. The developed surface MIPs not only showed imprinting factor (IF = 4) with reusability upon 5 cycles, but also capability of extracting lysozyme from egg-white directly. The MIPs showed controlled release of extracted lysozyme triggered by the NIR-light responsive property of the PDA layer. Moreover, the released lysozyme possesses good bioactivity, evidenced by efficient decomposition of micrococcus bacterial cell wall.
