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Gasoline-station workers in Brazil: Benzene exposure; Genotoxic and immunotoxic effects
Katia Soares da Poça1, Isabela Giardini1, Paula Vieira Baptista Silva1
1Área Técnica Ambiente, Trabalho e Câncer, Instituto Nacional de Câncer José Alencar Gomes da Silva - INCA, Rua Marquês do Pombal, 125/5º andar - Centro, Rio de Janeiro, RJ, CEP 20230-240, Brazil; Laboratório de Mutagênese Ambiental, Departamento de Bioquímica, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro (UNIRIO) - Rua Frei Caneca, 94/4º andar - Centro, Rio de Janeiro. CEP 20211-010, Brazil.
Abstract:
Chronic exposure to benzene is a risk factor for hematological malignancies. Gasoline-station workers are exposed to benzene in gasoline, via both inhalation and dermal contact (attendants and managers) or inhalation (workers in the on-site convenience stores and offices). We have studied the exposure of these workers to benzene and the resulting genotoxic and immunotoxic effects. Levels of urinary trans, trans-muconic acid were higher among gasoline-station workers than among office workers with no known exposure to benzene (comparison group). Among the exposed workers, we observed statistically significant biological effects, including elevated DNA damage (comet assay); higher frequencies of micronuclei and nuclear buds (CBMN assay); lower levels of T-helper lymphocytes and naive Th lymphocytes; lower CD4 / CD8 ratio; and higher levels of NK cells and memory Th lymphocytes. Both groups of exposed workers (inhalation and inhalation + dermal routes) showed similar genotoxic and immunotoxic effects.
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