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Related Experiment Video

Updated: Nov 8, 2025

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

An update on molecular counting in fluorescence microscopy.

Johan Hummert¹, Stanimir Asenov Tashev¹, Dirk-Peter Herten¹

¹College of Medical and Dental Sciences & School of Chemistry, University of Birmingham, Birmingham, UK; Centre of Membrane Proteins and Receptors (COMPARE), Universities of Birmingham and Nottingham, UK.

The International Journal of Biochemistry & Cell Biology

|April 18, 2021

Summary

This summary is machine-generated.

Accurate protein counting in cells is crucial for understanding cell signaling. New calibration methods using single-molecule microscopy improve the reliability of quantifying protein complexes and receptor clusters.

Keywords:

Calibration standards Oligomerization Protein complexes Quantitative microscopy

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Last Updated: Nov 8, 2025

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Published on: July 6, 2018

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

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Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Quantitative assessment of protein complexes, like receptor clusters in cellular signaling, is vital.
Single-molecule fluorescence microscopy enables protein copy number quantification in cellular structures.
Robust calibration protocols are increasingly important due to photophysical properties and labeling efficiencies.

Purpose of the Study:

To provide an update on recent protein counting methods.
To focus on novel calibration protocols for molecular counting.
To discuss calibration samples and challenges in protein counting experiments.

Main Methods:

Review of recent methods for protein counting.
Focus on novel calibration protocols.
Discussion of calibration samples and experimental challenges.

Main Results:

Identification of challenges in molecular counting experiments.
Presentation of potential approaches to address these challenges.
Update on recent advancements in protein counting techniques.

Conclusions:

Accurate protein quantification relies on robust calibration.
Novel calibration protocols are essential for reliable molecular counting.
Recent applications offer solutions for challenges in protein counting.