MXene-based enzymatic sensor for highly sensitive and selective detection of cholesterol

Area of Science:

• Materials Science
• Nanotechnology
• Biochemistry

Background:

• MXene (Ti3C2Tx) possesses advantageous properties like high surface area, biocompatibility, and conductivity.
• Chit/ChOx/Ti3C2Tx nanocomposite offers a promising matrix for enzyme immobilization and enhanced electron transfer.

Purpose of the Study:

• To develop a highly sensitive and selective electrochemical biosensor for cholesterol detection.
• To investigate the performance of a novel nanocomposite material for biosensing applications.

Main Methods:

• Fabrication of a Chit/ChOx/Ti3C2Tx nanocomposite via self-assembly.
• Characterization using SEM, CV, EIS, and DPV techniques.
• Development of a one-step dip-coating method for biosensor fabrication.

Main Results:

• The biosensor exhibited a linear response to cholesterol from 0.3 to 4.5 nM.
• Achieved a low detection limit of 0.11 nM and high sensitivity of 132.66 μA nM−1 cm−2.
• Demonstrated favorable selectivity, stability, and practicability in real samples.

Conclusions:

• The developed MXene-based biosensor offers a sensitive and reliable platform for cholesterol monitoring.
• The unique properties of the Chit/ChOx/Ti3C2Tx nanocomposite contribute to enhanced biosensing performance.
• This biosensor shows significant potential for practical applications in diagnostics and food safety.