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11:03
Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
Published on: December 22, 2014
Transgenes as probes for active chromosomal domains in mouse development
N D Allen1, D G Cran, S C Barton
1Department of Molecular Embryology, Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
Nature
|June 30, 1988
Summary
Transgenes reveal active chromosomal regions influencing gene expression during development. This tool maps gene activity by observing unique expression patterns in each transgenic organism.
Area of Science:
- Developmental Biology
- Genetics
- Epigenetics
Background:
- Embryonic development relies on precise gene expression timing and location.
- Active chromosomal domains can influence gene expression patterns.
- Transgenes integrating randomly can reveal these chromosomal influences via position effects.
Purpose of the Study:
- To systematically explore position effects on transgene expression.
- To utilize a specific transgene construct (HSV-TK-lacZ) to probe chromosomal activity.
- To establish transgenes as tools for mapping active genomic regions.
Main Methods:
- Systematic exploration of position effects using a weak promoter transgene (HSV-TK-lacZ).
- Analysis of lacZ expression patterns in transgenic fetuses.
- Assessment of transgene transmission across generations.
Main Results:
- Each transgenic fetus exhibited a unique lacZ staining pattern, indicating position-dependent expression.
- Transgene expression was dictated by chromosomal position, not construct-specific elements.
- The transgene was faithfully transmitted to subsequent generations.
Conclusions:
- Transgenes are powerful tools for identifying active chromosomal regions.
- This method can map changes in gene expression during development and adult life.
- Potential to identify endogenous genes involved in organogenesis and pattern formation.
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