Applicability of Superheated Water Extraction for the Quantification of Pesticide Residues in Leafy Vegetables

Superheated water extraction (SWE) of pesticide residues in a cabbage sample was demonstrated. The recovery yields of several relatively polar pesticides (log P_ow < 3) by the spike-and-recovery method at 100°C were acceptable. Increasing the extraction temperature up to 150°C led to enhanced extraction efficiency except for pesticides that induced degradation. The recovery yields of some target pesticides having log P_ow values of 3.5 - 4 were effectively enhanced by increasing the number of extraction cycles. The observed concentration of fenitrothion in a cabbage sample was comparable with those by the official Japanese analytical method. These results suggested SWE is potentially suitable for the extraction of different relatively high-to-medium polarity pesticides.