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Updated: Nov 8, 2025

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
Published on: September 8, 2023
Anomalous differential polarized phase angles for two-photon excitation with isotropic depolarizing rotations
Joseph R Lakowicz1, Ignacy Gryczynski1, Eva Danielsen1
1University of Maryland School of Medicine, Center for Fluorescence Spectroscopy, Department of Biological Chemistry, 660 West Redwood Street, Baltimore, MD 21201, USA.
Abstract:
We describe frequency-domain measurements of the anisotropy decay of 1,6-diphenylhexatriene resulting from one- and two-photon excitation. For two-photon excitation, the phase shifts (Δ) between the horizontally and vertically polarized components of the decay exceed the absolute maximum of 30° possible for one-photon excitation, and values of Δ as large as 37° were observed for 1,6-diphenylhexatriene in triacetin. These results are explained by the increased orientation of the photoselected population for two- as compared to one-photon excitation.
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