Related Experiment Video
Updated: Nov 8, 2025
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
Published on: May 16, 2012
circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21
Daoyuan Wang1,2, Shuxian Yan2, Lihui Wang1
1Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Background:
There is more and more evidence showed that circRNAs played essentially role in the regulation of various biological processes. The role of circSLC8A1 in prostate cancer (PCa) is yet little known.
Methods:
The CircSLC8A1 expression in human prostate cancer was measured by qRT-PCR. The interplay between the specific circRNA, miRNA, and mRNA was investigated by RT-PCR and luciferase reporter assay. Through transient transfection of siRNA, the impacts of circSLC8A1 on PCa were discussed. Cell cycle evaluation, transwell assay, and CCK-8 assay were employed to determine its biological influences.
Results:
In this study, our data revealed that circSLC8A1 was downregulated in PCa tissues and cells. The reduction of circSLC8A1 resulted in the inhibition of cell proliferation and migration. In mechanism, circSLC8A1 exhibited a direct interaction with miR-21 and displayed as a miRNA sponge to inhibit PCa progression. The functional analysis revealed that the circSLC8A1/miR-21 axis may regulate the cell proliferation, angiogenesis, cell migration, epithelial to mesenchymal transition, MAPK signaling pathway, and chemokine signaling pathway.
Conclusions:
CircSLC8A1 functioned as an inhibitor of neoplasm via modulating the miR-21 and might serve as a prospective target for the treatment of PCa.
Insights
Circular RNA SLC8A1 (circSLC8A1) is downregulated in prostate cancer (PCa), inhibiting tumor progression by interacting with miR-21. This suggests circSLC8A1 is a potential therapeutic target for PCa treatment.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Circular RNAs (circRNAs) are increasingly recognized for their regulatory roles in biological processes.
- The specific function of circRNA SLC8A1 (circSLC8A1) in prostate cancer (PCa) remains largely unexplored.
Purpose of the Study:
- To investigate the role and mechanism of circSLC8A1 in prostate cancer.
- To determine if circSLC8A1 can serve as a potential therapeutic target for PCa.
Main Methods:
- Quantitative reverse transcription PCR (qRT-PCR) to measure circSLC8A1 expression.
- RT-PCR and luciferase reporter assays to elucidate molecular interactions.
- Cell cycle, transwell, and CCK-8 assays to assess biological functions.
- siRNA transfection to study circSLC8A1's impact on PCa.
Main Results:
- circSLC8A1 expression was significantly downregulated in PCa tissues and cells.
- Reduced circSLC8A1 inhibited prostate cancer cell proliferation and migration.
- circSLC8A1 acts as a miRNA sponge for miR-21, suppressing PCa progression.
- The circSLC8A1/miR-21 axis influences cell proliferation, angiogenesis, migration, EMT, and MAPK/chemokine signaling pathways.
Conclusions:
- circSLC8A1 functions as a tumor suppressor in prostate cancer by modulating miR-21.
- circSLC8A1 represents a promising therapeutic target for prostate cancer treatment.
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