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High spatial and temporal resolution synthetic aperture phase microscopy.

Cheng Zheng1,2, Di Jin3, Yanping He1

  • 1The Chinese University of Hong Kong, Department of Biomedical Engineering, Hong Kong, China.

Advanced Photonics
|April 19, 2021
PubMed
Summary
This summary is machine-generated.

A novel optical microscopy technique, high spatial and temporal resolution synthetic aperture phase microscopy (HISTR-SAPM), doubles lateral resolution to 260 nm. This advanced imaging method enables nanoscale observation of materials and live cells with millisecond-level temporal resolution.

Keywords:
cell dynamics observationlabel-free imagingmaterial inspectionquantitative phase microscopy

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Area of Science:

  • Optical microscopy
  • Nanotechnology
  • Biophysics

Background:

  • Wide-field coherent imaging techniques often face limitations in achieving both high lateral resolution and fast temporal acquisition.
  • Improving the resolution of optical microscopy is crucial for detailed analysis of nanoscale structures and dynamic processes.

Purpose of the Study:

  • To introduce and validate a new optical microscopy technique, high spatial and temporal resolution synthetic aperture phase microscopy (HISTR-SAPM).
  • To demonstrate HISTR-SAPM's capability to enhance lateral resolution and temporal performance for advanced imaging applications.

Main Methods:

  • HISTR-SAPM utilizes digital micromirror devices for high-speed, stable alteration of illumination beam angles.
  • An off-axis interferometer captures scattered complex fields from the sample.
  • Reconstruction algorithms process the captured data to generate high-resolution phase images.

Main Results:

  • Achieved a twofold increase in lateral resolution, reaching approximately 260 nm under plane wave illumination.
  • Demonstrated millisecond-level temporal resolution, enabling the capture of dynamic events.
  • Successfully mapped height profiles of subwavelength photonic structures and resolved features with 198 nm linewidth and 132 nm gap.
  • Quantified nanoscale dynamics in live cells, including red blood cell membrane fluctuations and subcellular dynamics.

Conclusions:

  • HISTR-SAPM offers a significant advancement in optical microscopy, providing superior spatial and temporal resolution.
  • The technique effectively mitigates laser speckle noise, enhancing image quality for dynamic biological samples.
  • HISTR-SAPM is poised to benefit diverse research fields, including material science and live-cell biology.