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tpr homologues activate met and raf
H W King1, P R Tempest, K R Merrifield
1Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Oncogene
|June 1, 1988
Summary
The tpr domain, found in the tpr-met oncogene, shares a sequence with the rat raf oncogene. This suggests the tpr domain plays a role in activating both met and raf oncogenes.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The human tpr-met oncogene is implicated in cancer development.
- Previous studies characterized parts of the tpr and met domains.
Purpose of the Study:
- To determine the complete primary sequence of the tpr domain.
- To investigate potential functional roles of the tpr domain in oncogenesis.
Main Methods:
- Isolation and sequencing of a cDNA clone encoding the tpr domain.
- Bioinformatic analysis comparing the tpr sequence to known oncogene sequences.
Main Results:
- The complete primary sequence of the tpr domain was determined.
- A significant sequence identity was found between the tpr domain and the 5-prime end of the activated rat raf oncogene.
- tpr-like sequences were identified in both the tpr-met and raf oncogenes.
Conclusions:
- The tpr domain's sequence is conserved and present in multiple oncogenes.
- The tpr domain likely contributes to the activation mechanisms of both met and raf oncogenes.
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