Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Voltaic/Galvanic Cells02:47

Voltaic/Galvanic Cells

60.3K
Spontaneous Chemical Reactions
Spontaneous redox reactions occur abundantly in nature. The chemical reaction occurring in a disposable AA battery powering our remote controls is one such example of a spontaneous redox reaction. Another example is the immersion of coiled copper wire into an aqueous silver nitrate solution. The reaction shows a gradual, visually impressive color change from colorless to bright blue and the formation of a grey precipitate on the copper wire. In this experiment,...
60.3K
Reduction of Alkenes: Asymmetric Catalytic Hydrogenation02:17

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation

3.6K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.6K
Redox Reactions01:24

Redox Reactions

57.1K
Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...
57.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Covalent Insertion of a Mn(Salen) Type Complex in Cross-Linked Protein Crystals: Design of an Enantioselective Artificial Epoxidase.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

From two-component enzyme complex to nanobiohybrid for energy-efficient water-gas shift reaction.

Chemical science·2025
Same author

Insights into the Role of the D-Cluster in [NiFe]-CODH from Rhodospirillum Rubrum.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same author

Soft-Matter Physics Provides New Insights on Myocardial Architecture: Automatic and Quantitative Identification of Topological Defects in the Trabecular Myocardium.

Journal of cardiovascular development and disease·2024
Same author

Characterization of the Intracellular Acidity Regulation of Brain Tumor Cells and Consequences for Therapeutic Optimization of Temozolomide.

Biology·2023
Same author

Generalization of the Ratiometric Method to Extend pH Range Measurements of the BCECF Probe.

Biomolecules·2023

Related Experiment Video

Updated: Nov 8, 2025

Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells
06:39

Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells

Published on: October 20, 2023

3.5K

Hybrid Amyloid-Based Redox Hydrogel for Bioelectrocatalytic H2 Oxidation.

Nicolas Duraffourg1, Maxime Leprince1, Serge Crouzy1

  • 1Univ. Grenoble Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, 38000, Grenoble, France.

Angewandte Chemie (International Ed. in English)
|April 19, 2021
PubMed
Summary

Researchers developed an artificial amyloid hydrogel for efficient electron transfer in bioelectrodes. This novel material enables enhanced electrocatalytic oxidation of hydrogen (H2) using hydrogenase enzymes.

Keywords:
bioelectrocatalysishybrid prion forming domainprotein nanowiresredox hydrogelssupramolecular polymers

More Related Videos

Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

17.0K
Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors
09:15

Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors

Published on: November 22, 2016

10.8K

Related Experiment Videos

Last Updated: Nov 8, 2025

Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells
06:39

Author Spotlight: Design and Evaluation of Au-Electroplated Carbon Fiber Cloth Electrodes for Hydrogen Peroxide Fuel Cells

Published on: October 20, 2023

3.5K
Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

17.0K
Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors
09:15

Iridium Oxide-reduced Graphene Oxide Nanohybrid Thin Film Modified Screen-printed Electrodes as Disposable Electrochemical Paper Microfluidic pH Sensors

Published on: November 22, 2016

10.8K

Area of Science:

  • Biomaterials Science
  • Electrochemistry
  • Protein Engineering

Background:

  • Efficient electron transfer is crucial for bioelectrochemical systems.
  • Developing stable and effective immobilization matrices for enzymes like hydrogenase is challenging.
  • Amyloid self-assembly offers a promising route for creating ordered biomaterials.

Purpose of the Study:

  • To design an artificial amyloid-based redox hydrogel for mediating electron transfer between [NiFeSe] hydrogenase and electrodes.
  • To create self-assembling redox-active protein nanofibrils for bioelectrode fabrication.
  • To evaluate the performance of the fabricated bioelectrodes for hydrogen oxidation.

Main Methods:

  • Synthesis of a hybrid redox protein by incorporating a benzyl methyl viologen moiety into a mutated HET-s prion protein domain.
  • Self-assembly of the hybrid protein into structurally homogenous nanofibrils.
  • Fabrication of bioelectrodes by immobilizing [NiFeSe] hydrogenase within the redox hydrogel on carbon electrodes via a pH jump.
  • Electrochemical characterization of the bioelectrodes for hydrogen (H2) electrocatalytic oxidation.

Main Results:

  • Structurally homogenous amyloid nanofibrils with aligned redox groups were successfully synthesized.
  • The redox hydrogel effectively immobilized hydrogenase under mild conditions.
  • Fabricated bioelectrodes achieved catalytic current densities up to 270 μA cm⁻² for H2 oxidation.
  • An overpotential of 0.33 V was observed at 45°C under quiescent conditions.

Conclusions:

  • An artificial amyloid-based redox hydrogel is a viable material for enzyme immobilization and electron transfer mediation.
  • The developed bioelectrodes demonstrate efficient electrocatalytic activity for hydrogen oxidation.
  • This approach offers a promising strategy for constructing advanced bioelectrochemical devices.