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Related Experiment Video

Updated: Nov 8, 2025

Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish
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Visualizing Ocular Morphogenesis by Lightsheet Microscopy Using rx3:GFP Transgenic Zebrafish.

Rebecca A Petersen1, Ann C Morris2

  • 1Department of Biology, University of Kentucky.

Journal of Visualized Experiments : Jove
|April 19, 2021
PubMed
Summary
This summary is machine-generated.

This study details a protocol for time-lapse imaging of zebrafish eye development using lightsheet microscopy. This method allows for in vivo visualization of ocular morphogenesis, aiding research into developmental processes and genetic or drug-induced perturbations.

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Area of Science:

  • Developmental Biology
  • Neuroscience
  • Genetics

Background:

  • Vertebrate eye development (oculogenesis) is a complex process involving precise cell coordination.
  • Time-lapse imaging offers in vivo insights into cellular behaviors during development.
  • Zebrafish are a suitable model due to conserved eye structure, external development, and optical transparency.

Purpose of the Study:

  • To provide a protocol for time-lapse imaging of ocular morphogenesis in zebrafish.
  • To utilize lightsheet microscopy for high-resolution, in vivo visualization of eye development.
  • To enable tracking of the eye primordium from optic vesicle to optic cup formation.

Main Methods:

  • Employing the transgenic zebrafish line Tg(rx3:GFP) to mark eye field cells.
  • Utilizing lightsheet microscopy for high-speed, low-phototoxicity imaging of ocular development.
  • Developing a protocol for embryo preparation, embedding, imaging, and data analysis.

Main Results:

  • High-resolution time-lapse imaging successfully tracks the eye primordium's development into the retina.
  • The protocol facilitates visualization of ocular morphogenesis in vivo.
  • The method allows for the study of developmental perturbations caused by genetic or pharmacological factors.

Conclusions:

  • The described protocol enables detailed in vivo study of zebrafish ocular morphogenesis.
  • Lightsheet microscopy is an effective tool for high-speed, high-resolution imaging of developmental processes.
  • This approach provides valuable data for understanding normal and perturbed eye development.