Expression and prognosis analyses of BUB1, BUB1B and BUB3 in human sarcoma
Related Experiment Video
Updated: Nov 8, 2025
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Zeling Long1, Tong Wu1, Qunyan Tian1
1Department of Orthopedics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Abstract:
Budding Uninhibited By Benzimidazoles are a group of genes encoding proteins that play central roles in spindle checkpoint during mitosis. Improper mitosis may lead to aneuploidy which is found in many types of tumors. As a key mediator in mitosis, the dysregulated expression of BUBs has been proven to be highly associated with various malignancies, such as leukemia, gastric cancer, breast cancer, and liver cancer. However, bioinformatic analysis has not been applied to explore the role of the BUBs in sarcomas. Herein, we investigate the transcriptional and survival data of BUBs in patients with sarcomas using Oncomine, Gene Expression Profiling Interactive Analysis, Cancer Cell Line Encyclopedia, Kaplan-Meier Plotter, LinkedOmics, and the Database for Annotation, Visualization and Integrated Discovery. We found that the expression levels of BUB1, BUB1B and BUB3 were higher in sarcoma samples and cell lines than in normal controls. Survival analysis revealed that the higher expression levels of BUB1, BUB1B and BUB3 were associated with lower overall and disease-free survival in patients with sarcomas. This study implies that BUB1, BUB1B and BUB3 are potential treatment targets for patients with sarcomas and are new biomarkers for the prognosis of sarcomas.
Insights
Budding Uninhibited By Benzimidazoles (BUBs) genes like BUB1, BUB1B, and BUB3 are overexpressed in sarcomas. Their elevated expression correlates with poorer survival, suggesting BUBs as potential therapeutic targets and prognostic biomarkers for sarcoma patients.
Area of Science:
- Molecular biology
- Cancer research
- Bioinformatics
Background:
- Budding Uninhibited By Benzimidazoles (BUBs) are crucial for the spindle checkpoint during mitosis.
- Mitotic errors can lead to aneuploidy, a hallmark of many cancers.
- Dysregulated BUB gene expression is linked to various malignancies, but their role in sarcomas is unexplored.
Purpose of the Study:
- To investigate the role of BUB genes in sarcomas using comprehensive bioinformatic analysis.
- To determine the prognostic significance of BUB gene expression in sarcoma patients.
Main Methods:
- Utilized multiple bioinformatic databases: Oncomine, GEPIA, CCLE, Kaplan-Meier Plotter, LinkedOmics, and DAVID.
- Analyzed transcriptional and survival data of BUB genes in sarcoma samples and cell lines.
- Correlated BUB gene expression levels with patient survival outcomes.
Main Results:
- BUB1, BUB1B, and BUB3 genes showed significantly higher expression in sarcoma tissues and cell lines compared to normal controls.
- Elevated expression of BUB1, BUB1B, and BUB3 was associated with decreased overall survival and disease-free survival in sarcoma patients.
Conclusions:
- BUB1, BUB1B, and BUB3 are potential therapeutic targets for sarcoma treatment.
- These BUB genes serve as novel biomarkers for predicting sarcoma prognosis.
