Cryo-EM structure of human ABCB8 transporter in nucleotide binding state
Shunjin Li1, Yue Ren1, Xuhang Lu1
1State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300350, China; College of Life Sciences, Nankai University, Tianjin 100094, China.
Abstract:
Human ATP-binding cassette transporter 8 of subfamily B (hABCB8) is an ABC transporter that located in the inner membrane of mitochondria. The ABCB8 is involved in the maturation of Fe-S and protects the heart from oxidative stress. Here, we present the cryo-EM structure of human ABCB8 binding with AMPPNP in inward-facing conformation with resolution of 4.1 Å. hABCB8 shows an open-inward conformation when ATP is bound. Unexpectedly, cholesterol molecules were identified in the transmembrane domain of hABCB8. Our results provide structural basis for the transport mechanism of the ABC transporter in mitochondria.
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