Mycorrhizal diversity in photosynthetic terrestrial orchids

•  Specific orchid-fungal associations are known for nonphotosynthetic orchids but fungal diversity in photosynthetic orchids is thought to be quite broad. Specific fungal associations will figure prominently in conservation efforts, while diverse associations may require less attention. We combined culture techniques with ITS and mtLSU sequences and phylogenetic analysis to determine the genetic diversity of mycorrhizal fungi associated with an evergreen, a spring-green, and a winter-green orchid and compared this diversity with that published for a nonphotosynthetic orchid. •  Mycorrhizal diversity in two of the three photosynthetic orchids was lower than for the nonphotosynthetic orchid. Mycorrhizal diversity in protocorms of the third species was also equal to, or less than, the fungal diversity associated with the nonphotosynthetic species, but adult fungal diversity was greater. •  We found that photosynthetic orchids do not necessarily have more diverse mycorrhizal associations than nonphotosynthetic orchids. Similarly, evergreen orchids do not necessarily have greater mycorrhizal diversity than seasonally green orchids. Thus, orchid mycorrhizal diversity may not be determined by adult photosynthetic capacity.