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Updated: Nov 8, 2025

Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions

Reliable Method for Assessing Seed Germination, Dormancy, and Mortality under Field Conditions

Published on: November 6, 2016

ABSCISIC ACID AND DORMANCY IN ROOT-NODULATED ALNUS GLUTINOSA (L.) GAERTN.

S H Watts¹, C T Wheeler¹, J R Hillman¹

¹Department of Botany, University of Glasgow, Glasgow G12 8QQ, UK.

The New Phytologist

|April 20, 2021

Summary

Abscisic acid (ABA) treatment induced resting bud formation in Alnus glutinosa, reducing leaf growth but not root or nodule biomass. ABA isomers accumulated differently in plant parts, with active forms concentrating in leaves and less active forms in roots.

Keywords:

Abscisic acid Alnus glutinosa dormancy root nodules

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Area of Science:

Plant Physiology
Plant Hormones
Plant Development

Background:

Abscisic acid (ABA) is a key plant hormone regulating various physiological processes, including growth and stress responses.
Understanding ABA's role in bud formation and its distribution within plant tissues is crucial for plant science.
Alnus glutinosa (common alder) is a woody plant species where ABA's effects on development can be studied.

Purpose of the Study:

To investigate the effects of exogenous abscisic acid (ABA) on the induction of resting buds in Alnus glutinosa.
To quantify the uptake and distribution of ABA and its isomers within different plant tissues.
To correlate ABA isomer accumulation with observed growth responses in leaves, roots, and nodules.

Main Methods:

Water culture plants of Alnus glutinosa were treated with 0.1 mol m⁻³ abscisic acid (ABA).
Plant growth parameters (leaf, root, nodule dry weights) were measured over 30 to 60 days.
Radioactive [2-¹⁴C] ABA was used to track uptake and distribution; ABA isomer levels (cis/trans, free/bound) were analyzed in various plant parts.

Main Results:

ABA treatment induced resting bud formation and reduced leaf dry weight by 40-45%, while root and nodule dry weights remained unaffected.
ABA was readily taken up by the root system, leading to significantly elevated ABA levels in treated plants.
ABA isomer distribution varied: biologically active cis-ABA accumulated in leaves and shoot apices, while less active trans-ABA dominated in roots and nodules.

Conclusions:

Exogenous ABA effectively induces resting bud formation and alters growth in Alnus glutinosa, with differential effects on various plant organs.
The accumulation of specific ABA isomers in different tissues correlates with localized growth responses, suggesting a role in regulating organ-specific development.
ABA's role in nodule growth and dormancy appears limited, as high levels of less active trans-ABA were found in nodules without significant growth inhibition.