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Observations on chloroplast starch content and synthesis in the intertidal marine alga Codium fragile (Suringar)
Michael L Williams1, A H Cobb1
1Department of Life Sciences, Trent Polytechnic, School of Science, Nottingham NG11 8NS, U.K.
Abstract:
A marked seasonal response was observed in the starch content of chloroplasts of Codium fragile sampled between November 1983 and November 1984. Chloroplast starch uniformly increased throughout the life-cycle of this alga, reaching a maximum in the summer months of approx. 5 mg glucose equivalents/109 plastids when C. fragile was in the reproductive phase. Chloroplast starch content was found to be inversely proportional to the concentration of stromal phosphate, decreasing linearly from 5 to 2 mg glucose equivalents/109 plastids over a range of stromal phosphate values of 0·75-1·5 μmol PO4 /109 plastids. Similarly, the net amount of starch synthesized over 3 h by isolated chloroplasts increased linearly with respect to initial chloroplast starch content. These observations are discussed in relation to the regulation of starch synthesis by variations in the stromal P1 : PGA ratio.
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