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Glutamate dehydrogenases in ectomycorrhizas of spruce (Picea excelsa L.) and beech (Fagus sylvatica L.)
1School of Biological Sciences, Murdoch University, Murdoch 6150, Western Australia.
Abstract:
The ammonia assimilation enzyme glutamate dehydrogenase as studied in extracts of spruce (Picea excelsa L.) roots, mycelium of a mycorrhizal fungus (Hebeloma sp.) and associated ectomycorrhizas. Evidence from enzyme reactions in crude extracts, electrophoretic patterns and immunological tests Using antibodies raised against purified NADP-GDH of Cenococcum geophilum Fr. consistently showed that Hebeloma NADP-dependent GDH was active in spruce ectomycorrhizas. Histochemical studies associated some NADP-GDH activity with the Hartig net. By contrast, the NADP-GDH fungal pathway was strongly suppressed in beech (Fagus sylvatica L.) associations with Hebeloma crustuliniforme (Bull. ex St Amans) Quél. and Paxillus involutus (Batsch ex Fr.) Fr.
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