VESICULAR-ARBUSCULAR MYCORRHIZA IMPROVE BOTH SYMBIOTIC N₂ FIXATION AND N UPTAKE FROM SOIL AS ASSESSED WITH A ¹⁵ N...

A technique using ¹⁵ N-labelled inorganic fertilizer was applied to estimate N₂ fixation by the forage legume Hedysarum coronarium L. and to ascertain the role of vesicular-arbuscular (VA) mycorrhizas in plant N nutrition throughout a growing season under field conditions. The absence of the specific Rhizobium for the forage legume in the test soil allowed us the use of ¹⁵ N methodology with the same legume as reference 'non-fixing' crop. At the first harvest, mycorrhizal inoculation behaved similarly to the phosphate addition in improving the percentage (70 %) and the total amount of N derived from fixation. But thereafter, mycorrhizal inoculation not only enhanced dry matter yield, N concentration and total N yield but also the amount of N derived from soil and from fixation, as compared with either phosphate-added or control plants. This indicated that mycorrhizas acted both by a P-mediated mechanism to improve N₂ fixation and by enhancing N uptake from soil. The latter agrees with recent findings by others that VA mycorrhizal hyphae can translocate and assimilate ammonium, a fact of physiological and ecological interest.