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Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
Published on: December 27, 2018
Chlorophyll fluorescence decay profiles of O3 -exposed spruce needles as measured by time-correlated single photon
E H Evans1, R G Brown2, A R Wellburn3
1School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L3 3AF.
Abstract:
Chlorophyll fluorescence decay profiles have been measured in the wavelength range 680-720 nm for needles from Norway spruce [Picea abies (L.) Karst] trees which have been exposed to ozone. All profiles required three exponential components of lifetime 100-150 ps, 400-600 ps and 3.5-5.0 ns to fit the experimental data. Compared to control samples, the ozone-treated needles exhibited a greater amount of the longest-lived chlorophyll fluorescence and a redistribution in intensity for both the other components from 720 + to 690 nm. These observations are interpreted in terms of disruption of energy transfer and break-up of light-harvesting complexes on exposure to ozone. The potential for use of the technique in monitoring forest decline is discussed.
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