Nitrate and ammonium uptake by maize: adaptation during relief from nitrogen suppression

Initial (0-1 h) net rates of nitrate and ammonium uptake from 200 μM NH₄ NO₃ were progressively increased as 8-d-old maize (Zea mays L.) seedlings, grown on 5 mM nitrate, were exposed to nitrogen-free solutions for up to 48 h. Further nitrogen deprivation to 72 h resulted in a decline in the nitrate uptake rate. Nitrate uptake rates of plants at all stages of nitrogen deprivation increased steadily during an 8 h exposure to 200 μM NH₄ NO₃ . The pattern of the response of ammonium uptake during the 8 h adaptation period was considerably different. In nitrogen-replete plants the ammonium uptake rate increased steadily, but deprivation of nitrogen for 12 h and longer resulted in complex responses in which the initial rate was followed by a decline, a subsequent increase, and another decline. The responses of the nitrate uptake system are considered to reflect a lifting of the suppressive effects of nitrate and a product of nitrate assimilation during nitrogen deprivation, a concomitant degradation of an induced component of the nitrate uptake system during that time, and reinduction of the uptake system during the adaptation period. The responses of the ammonium uptake system are considered to reflect the interplay of suppression by a product of ammonium assimilation, the accumulation of root ammonium and associated ammonium efflux, and a stimulation by ammonium of its own uptake. As a consequence of the differential responses of the two uptake systems, nitrate and ammonium uptake rates were positively correlated, largely independent, or negatively correlated as the plants progressed through the 8 h adaptation period.