Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nonenzymatic sequence-specific methyl transfer to single-stranded DNA.

B L Iverson1, P B Dervan

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125.

Proceedings of the National Academy of Sciences of the United States of America
|July 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploiting the interactions of aromatic units for folding and assembly in aqueous environments.

Chemical communications (Cambridge, England)·2016
Same author

Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays.

Clinical and vaccine immunology : CVI·2006
Same author

Passive protection against anthrax by using a high-affinity antitoxin antibody fragment lacking an Fc region.

Infection and immunity·2005
Same author

Models of higher-order structure: foldamers and beyond.

Current opinion in chemical biology·2001
Same author

Production of correctly folded Fab antibody fragment in the cytoplasm of Escherichia coli trxB gor mutants via the coexpression of molecular chaperones.

Protein expression and purification·2001
Same author

Molecular recognition of DNA by small molecules.

Bioorganic & medicinal chemistry·2001
Same journal

The TaMYB55-TaSnRK1α1-TabZIP9 module confers heat stress tolerance in wheat.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Superstatistics approach to turbulent circulation fluctuations.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

A molecular timescale for evolution of cobamide biosynthesis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Pierre Chambon, a pioneer of molecular biology and gene regulation in eukaryotes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Granulosa cell glycogen fuels the avascular corpus luteum.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Synthetic essentiality of TRAIL/TNFSF10 in VHL-deficient renal cell carcinoma.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Researchers developed a novel DNA modification method using a modified deoxyuridine triphosphate. This enables sequence-specific DNA cleavage at guanine sites, paving the way for new DNA methyltransferase designs.

Area of Science:

  • Chemical Biology
  • Molecular Biology
  • Synthetic Chemistry

Background:

  • DNA modification and cleavage are crucial for molecular biology and synthetic chemistry.
  • Enzymatic methods are common for DNA manipulation, but nonenzymatic approaches offer alternative strategies.
  • Understanding DNA-protein interactions and chemical reactivity is key to developing novel DNA-targeting tools.

Purpose of the Study:

  • To investigate the incorporation of a modified 2'-deoxyuridine 5'-triphosphate into DNA.
  • To explore the potential of this modified nucleoside for sequence-specific DNA cleavage.
  • To elucidate the mechanism of methyl-group transfer and its application in DNA modification.

Main Methods:

  • Incorporation of 5-methylthioether-modified 2'-deoxyuridine 5'-triphosphate into a primer-template DNA complex using Klenow enzyme.

Related Experiment Videos

  • Chemical activation of the modified nucleoside with cyanogen bromide (CNBr).
  • Subsequent treatment with piperidine to induce DNA cleavage and analysis of the reaction mechanism.

    • Main Results:

    • Successful incorporation of the modified nucleoside into DNA.
    • Demonstration of sequence-specific DNA cleavage predominantly at guanine residues.
    • Identification of a methyl-group transfer mechanism from the modified deoxyuridine to the N-7 position of guanine.

    Conclusions:

    • The modified deoxyuridine enables nonenzymatic, sequence-specific DNA cleavage.
    • The mechanism involves a novel sulfur-to-nitrogen methyl-group transfer.
    • This research opens possibilities for designing synthetic, sequence-specific DNA methyltransferases.