Related Experiment Video

Updated: Nov 8, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.5K

Clustered Regularly Interspaced Short Palindromic Repeats System of Genome Engineering in Embryos to Repair Genes

Firoozeh Niazvand, Zohre Fathinezhad, Narjes Alfuraiji

    Journal of Biomedical Nanotechnology
    |April 20, 2021
    PubMed

    More Related Videos

    Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells09:04

    Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

    Published on: September 25, 2019

    8.5K
    CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus AAV and 2-Cell Embryo Electroporation08:24

    CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus AAV and 2-Cell Embryo Electroporation

    Published on: March 15, 2024

    2.3K

    Related Concept Videos

    CRISPR/Cas9 Genome Editing01:28

    CRISPR/Cas9 Genome Editing

    The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
    851
    CRISPR01:59

    CRISPR

    Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
    54.2K
    Conservative Site-specific Recombination and Phase Variation02:53

    Conservative Site-specific Recombination and Phase Variation

    Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
    The recognition sites for Cre recombinase called LoxP...
    6.3K
    Homologous Recombination02:31

    Homologous Recombination

    The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
    58.4K
    In-vitro Mutagenesis01:16

    In-vitro Mutagenesis

    To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
    15.6K