Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

8.4K
Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hybrid solid-liquid optics enable scalable, high-resolution light-sheet microscopy across diverse immersion media.

Nature biotechnology·2026
Same author

Glial cell states bias the regeneration of neuron types across the newt life cycle.

bioRxiv : the preprint server for biology·2026
Same author

Evolutionary origins and transcriptomic innovations of vertebrate Cajal-Retzius cells.

Current biology : CB·2026
Same author

Distinct radial glia subtypes regulate midbrain dopaminergic neuron development.

Nature neuroscience·2026
Same author

Sustained HIV-1 remission after heterozygous CCR5Δ32 stem cell transplantation.

Nature·2025
Same author

Specialized signaling centers direct cell fate and spatial organization in a mesodermal organoid model.

Science advances·2025
Same journal

Frontier AI companies as biotech acquirers.

Nature biotechnology·2026
Same journal

Author Correction: Sustained nitric oxide production by engineered E. coli remodels the tumor microenvironment and potentiates immunotherapy.

Nature biotechnology·2026
Same journal

Quantum computing in transition.

Nature biotechnology·2026
Same journal

Author Correction: Improved RNA base editing with guide RNAs mimicking highly edited endogenous ADAR substrates.

Nature biotechnology·2026
Same journal

Unlocking the chemical potential of filamentous fungi using prime editing.

Nature biotechnology·2026
Same journal

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
See all related articles

Related Experiment Video

Updated: Nov 8, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.2K

Spatial tissue profiling by imaging-free molecular tomography.

Halima Hannah Schede1, Christian G Schneider1,2, Johanna Stergiadou3,4

  • 1Brain Mind Institute, School of Life Sciences, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Nature Biotechnology
|April 20, 2021
PubMed
Summary
This summary is machine-generated.

Spatial transcriptomics techniques (STRP-seq) enable imaging-free tissue analysis. This method reconstructs spatial gene expression data from sliced samples, revealing lizard brain molecular anatomy.

More Related Videos

Hybrid µCT-FMT imaging and image analysis
13:45

Hybrid µCT-FMT imaging and image analysis

Published on: June 4, 2015

13.4K
Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
06:47

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

2.3K

Related Experiment Videos

Last Updated: Nov 8, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.2K
Hybrid µCT-FMT imaging and image analysis
13:45

Hybrid µCT-FMT imaging and image analysis

Published on: June 4, 2015

13.4K
Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples
06:47

Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

2.3K

Area of Science:

  • Genomics
  • Neuroscience
  • Molecular Biology

Background:

  • Spatially resolved omics profiling is advancing, but new methods often require specialized equipment.
  • Existing techniques for spatial transcriptomics can be complex and instrument-dependent.

Purpose of the Study:

  • To develop an imaging-free framework for spatial omics profiling.
  • To adapt a low-input RNA sequencing protocol into a spatial transcriptomics technique (STRP-seq).
  • To investigate the molecular anatomy of the reptilian brain.

Main Methods:

  • Developed an imaging-free framework involving sample sectioning for image reconstruction.
  • Implemented the framework to create STRP-seq, a spatial transcriptomics method.
  • Applied STRP-seq to profile the spatial transcriptome of mouse and bearded dragon brains.

Main Results:

  • Successfully profiled the spatial transcriptome of the mouse brain.
  • Revealed the molecular anatomy of the Australian bearded dragon telencephalon.
  • Provided evidence for significant regionalization in the reptilian pallium and subpallium.

Conclusions:

  • STRP-seq offers a versatile imaging-free approach for spatial transcriptomics.
  • The technique can be adapted for various omics modalities.
  • The study elucidated the regional organization of the reptilian brain, contributing to comparative neuroanatomy.