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Updated: Nov 8, 2025

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Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
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Capturing protein droplets: label-free visualization and detection of protein liquid-liquid phase separation with an
Chu-Qiao Liang1, Lin Wang, Yun-Yi Luo
1Key Lab of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University Beijing, 100084, P. R. China. liym@mail.tsinghua.edu.cn.
Abstract:
We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of the protein condensed phase based on the lifetime of the DroProbe was also developed.

