Standard operating procedure for NanoTemper Monolith measurements

Area of Science:

• Biophysical techniques
• Biochemistry
• Molecular interactions

Background:

• Microscale thermophoresis (MST) is a widely used technique for analyzing molecular interactions.
• Standardized protocols are crucial for reproducible and reliable MST results.
• Accurate execution of MST experiments ensures data integrity.

Purpose of the Study:

• To provide a comprehensive, step-by-step standard operating procedure (SOP) for conducting microscale thermophoresis (MST) experiments.
• To guide researchers in the proper use of NanoTemper Technology GmbH Monolith instruments for MST analysis.
• To ensure consistent and high-quality data acquisition in temperature-related intensity change (TRIC) assays.

Main Methods:

• Detailed step-by-step instructions for setting up and running an MST experiment.
• Guidance on sample preparation, instrument calibration, and data collection using Monolith instruments.
• Explanation of temperature-related intensity change (TRIC) principles in the context of MST.

Main Results:

• A standardized protocol for reproducible MST experiments.
• Method for accurate measurement of molecular binding affinities and interactions.
• Optimized workflow for utilizing NanoTemper Monolith instruments.

Conclusions:

• Adherence to this SOP ensures reliable and accurate microscale thermophoresis (MST) data.
• This protocol facilitates efficient and effective use of NanoTemper Monolith instruments for molecular interaction studies.
• Standardized MST procedures enhance the reproducibility of biophysical measurements.