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Conditional destabilization of the TPLATE complex impairs endocytic internalization.

Jie Wang1,2, Klaas Yperman1,2, Peter Grones1,2

  • 1Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium.

Proceedings of the National Academy of Sciences of the United States of America
|April 20, 2021
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Researchers developed a new conditional tool to reversibly impair plant endocytosis. This method uses a modified TPLATE allele, allowing precise control over cellular processes vital for plant growth and defense.

Keywords:
ArabidopsisTPLATE complexautophagyclathrin-mediated endocytosistemperature-sensitive mutant

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Area of Science:

  • Plant Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Endocytosis is crucial for plant development, hormone signaling, nutrient uptake, and pathogen defense.
  • Existing methods to manipulate plant endocytosis are limited, hindering research and applications.

Purpose of the Study:

  • To develop a novel, conditional tool for reversibly impairing endocytosis in plants.
  • To investigate the role of the TPLATE complex (TPC) in endocytosis and its potential for specific modulation.

Main Methods:

  • Generated a partially functional TPLATE allele by substituting its most conserved domain.
  • Utilized short-term heat treatment to induce TPLATE delocalization and destabilize the TPC.
  • Assessed the impact on endocytosis by monitoring FM uptake and cargo accumulation.

Main Results:

  • The modified TPLATE allele, upon heat treatment, reversibly impaired endocytosis.
  • Heat treatment caused TPLATE to delocalize from the plasma membrane to cytoplasmic aggregates.
  • Known endocytic cargoes accumulated at the plasma membrane, confirming impaired endocytosis.
  • The tool specifically modulated endocytosis without affecting autophagy.

Conclusions:

  • A conditional tool was successfully developed to specifically and reversibly impair plant endocytosis.
  • This tool provides a valuable method for studying endocytosis-related processes in plants.
  • The findings offer new possibilities for manipulating plant cellular functions.