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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Related Experiment Video

Updated: Nov 8, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Locus-Specific Genomic DNA Purification Using the CRISPR System: Methods and Applications.

Hirotaka Fujita1, Toshitsugu Fujita1, Hodaka Fujii1

  • 1Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, Aomori, Japan.

The CRISPR Journal
|April 20, 2021
PubMed
Summary
This summary is machine-generated.

CRISPR technology allows scientists to purify specific DNA regions, aiding in the study of gene regulation and disease. This method identifies molecules interacting with targeted genomic sites for deeper biological insights.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Chromatin interactions are crucial for cellular chromosomal functions.
  • Understanding molecular compositions at genomic loci is key to gene regulation and disease pathogenesis.
  • Abnormal gene regulation can lead to various disorders.

Purpose of the Study:

  • To provide an overview of CRISPR-based DNA purification methodologies.
  • To highlight recent applications of this technology.
  • To deepen the understanding of molecular interactions at specific genomic regions.

Main Methods:

  • Utilizing the clustered regularly interspaced short palindromic repeats (CRISPR) system for locus-specific DNA purification.
  • Isolating target genomic regions for the identification of bound interacting molecules.
  • Reviewing established and emerging CRISPR-based DNA purification techniques.

Main Results:

  • Demonstrated the capability of CRISPR systems to enable targeted isolation of genomic DNA.
  • Identified bound interacting molecules at purified genomic loci.
  • Presented a comprehensive overview of CRISPR-based DNA purification strategies.

Conclusions:

  • CRISPR-based DNA purification is a powerful tool for investigating molecular interactions at specific genomic sites.
  • This methodology offers significant applications in understanding gene regulation and disease mechanisms.
  • Further research and application of CRISPR DNA purification will advance molecular biology and genomics.