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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

Updated: Nov 8, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
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Identification of Dysregulated microRNAs in Glioma Using RNA-sequencing.

Chang Liu1, Ying-Ying Ge2, Xiao-Xun Xie2

  • 1Department of Neurosurgery, the First Affiliated Hospital, Guangxi Medical University, Nanning, 530021, China.

Current Medical Science
|April 20, 2021
PubMed
Summary

Dysregulated microRNAs (miRNAs) are key in glioma progression. This study identified specific miRNAs in low-grade glioma and glioblastoma, offering potential molecular targets for novel glioma therapies.

Keywords:
RNA-sequencinggliomamicroRNA

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Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Genomics

Background:

  • Glioma is a common malignant brain tumor with high relapse rates.
  • Current treatments like surgery and chemoradiotherapy have limitations.
  • MicroRNAs (miRNAs) are implicated in glioma development and progression.

Purpose of the Study:

  • To investigate the expression profile of miRNAs in glioma tissues.
  • To identify differentially expressed miRNAs (DEmiRNAs) and their target genes.
  • To provide a reference for potential molecular-targeting therapies for glioma.

Main Methods:

  • RNA sequencing (RNA-seq) to profile miRNA expression in normal, low-grade glioma (LGG), and glioblastoma (GBM) tissues.
  • Bioinformatic analysis including target gene prediction, Gene Ontology (GO) enrichment, and KEGG pathway analysis.
  • Validation of selected DEmiRNAs using real-time quantitative PCR (qRT-PCR).

Main Results:

  • Significant differences in miRNA expression were observed between glioma and normal brain tissues.
  • RNA-seq identified numerous up- and down-regulated miRNAs in both LGG and GBM.
  • Bioinformatics analysis revealed DEmiRNA targets involved in cell metabolism and development.
  • qRT-PCR confirmed specific miRNAs (e.g., miR-10b-5p, miR-184) with altered expression in glioma.

Conclusions:

  • Dysregulated miRNAs play a crucial role in glioma pathogenesis.
  • Several identified DEmiRNAs represent potential diagnostic biomarkers.
  • These dysregulated miRNAs may serve as promising molecular targets for future glioma treatments.